1 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tube as serum from a plain red top tube. Freeze.
Note: Collect all samples at the same time of day, as there is a diurnal variation of pinp with the values being higher at night.