0.3 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tube as serum from a plain red top tube. Refrigerate.
Note: when ordering multiple IgE specific allergens, the recommended specimen volume is:
For 2-24 allergens: minimum 2 mL serum.
For 25-40 allergens: minimum 3-4 mL serum.