Lupus Anticoagulant Screen, Reflex Conf

Test Code: TD_1485
CPT Code(s): 85610, 85730, 85613
When Confirmation Testing Performed, Additional Codes May Apply As Follows:
85670,85635,85525,85732,85613 X2,85598

$90

Test Details

Alternative Name(s)

Partial Thromboplastin Time – Lupus Anticoagulant: PTT-LA, Lupus PTT
Dilute Russell’s Viper Venom Time: Russel Venom, DRVVT

Test Includes

Prothrombin Time
Partial Thromboplastin Time – Lupus Anticoagulant
Dilute Russell’s Viper Venom Time

Methodology

Automated Electromechanical Clot Detection

Rejection Criteria

Hemolysis
Lipemia
Thawed samples

Reference Range(s)

By Report

Specimen Requirements

Preferred Specimen

Sodium Citrate Light Blue Top Tube

Minimum Volume

2.5 mL Plasma

Sample Stability

Screen: 4 hours room temperature
Unacceptable refrigerated
1 month frozen at -20 degrees C
Prothrombin Time: 4 hours room temperature
Unacceptable refrigerated
1 month frozen at -20 degrees C
Partial Thromboplastin Time – Lupus Anticoagulant: 8 hours room temperature
Unacceptable refrigerated
1 month frozen at -20 degrees C
Dilute Russell’s Viper Venom Time: 4 hours room temperature
Unacceptable refrigerated
1 month frozen at -20 degrees C

Transport Temperature

Critical Frozen

Collection Instructions

1. Fill a light blue top tube as far as vacuum will allow and mix by gentle inversion five to six times. The exact ratio of 1 part sodium citrate to 9 parts blood must be maintained. Inadequate filling of the sample tube will alter this ratio and may lead to inaccurate results.
2. Patients who have hematocrit values above 55% should have the anticoagulant adjusted to maintain the 1 to 9 ratio.
3. To obtain platelet-poor plasma, immediately centrifuge sample at 1500 RCFs for 15 minutes, then pipette the supernatant plasma and transfer to a plastic transport tube.
4. Re-centrifuge plasma in the plastic transport tube at 1500 RCFs for 15 minutes to ensure that no red cells are present.
5. Pipette the top two-thirds of the supernatant plasma and transfer to another plastic transport tube.
6. Immediately freeze the plasma at -20 degrees C or lower for transport.
7. When multiple tests are ordered, submit separate tubes for this test.

Alternative Specimen

N/A