1. Obtain an adequate sampling from the cervix using spatula/brush or broom.
2. Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix.
3. Push gently and rotate the broom in a clockwise direction five (5) times.
4. Rinse the broom into the vial by pushing the broom into the bottom of the vial ten (10) times, forcing the bristles apart.
5. Swirl the broom vigorously to further dislodge material.
6. Discard the collection device. Do not leave collection device in the vial.
7. Tighten the cap so that the torque line on the cap passes the torque line on the vial.
8. Record the patient name and id number on the vial. The sample will be returned if it is received without proper labeling. Also record the patient name and medical history on the cytology requisition.
Submit the sample to the laboratory at room temperature.