HLA B-27 by Flow, Reflex PCR

Test Code: TD_4864

CPT Code(s): 86812

$40

Test Details

Alternative Name(s)

N/A

Test Includes

HLA b-27 by Flow Cytometry
Will reflex to HLA-B27 by PCR for confirmation when result is indeterminate.

Methodology

Flow cytometry

Rejection Criteria

Samples collected in lithium heparin tube
Hemolysis

Reference Range(s)

Not present
Will reflex to HLA-B27 by PCR for confirmation when result is indeterminate.

Specimen Requirements

Preferred Specimen

EDTA (lavender top) tube

Minimum Volume

2 mL whole blood

Sample Stability

3 days room temperature
Note: refrigerated samples are unacceptable; however samples refrigerated for up to 72 hours will be tested and results individually assessed by a pathologist. Results may be rejected, requiring sample recollection.

Transport Temperature

Critical Room Temperature

Collection Instructions

Fill EDTA tube and gently mix by inverting the tube 8-10 times. Critical Room Temperature.

Alternative Specimen

4 mL sodium heparin (green top) whole blood. Critical Room Temperature (CRT).
Note: confirmatory testing cannot be performed with sodium heparin (green top) results obtained by flow cytometry.