1 mL serum from a plain red top tube. Allow sample to clotin an upright position for at least 60 minutes, thencentrifuge sample and transfer serum to a plastic transporttube within 2 hours of collection. Clearly label tube asserum from a plain red top tube. Refrigerate.Note: Do not collect samples from patients receiving therapywith high biotin doses (>5 mg/day) until at least 8 hoursfollowing the last biotin administration.