Factor V Activity

Test Code: TD_4844
CPT Code(s): 85220

$59

Test Details

Alternative Name(s)

Coagulation factor V
Factor 5 activity

Test Includes

Factor V Activity

Methodology

Clot Detection

Rejection Criteria

Hemolysis
Thawed samples

Reference Range(s)

“FactorV

Specimen Requirements

Preferred Specimen

Completely filled Citrate Blue Top Tube

Minimum Volume

2 mL Frozen Plasma

Sample Stability

4 hours room temperature
4 hours refrigerated
2 weeks frozen

Transport Temperature

Critical Frozen (CFZ)

Collection Instructions

1. Fill a light blue top tube as far as vacuum will allow and mix by gentle inversion five to six times. The exact ratio of 1 part sodium citrate to 9 parts blood must be maintained. Inadequate filling of the sample will alter this ratio and may lead to inaccurate results.
2. Patients who have hematocrit values above 55% should
have the anticoagulant adjusted to maintain the 1 to 9 ratio.
3. To obtain platelet-poor plasma, immediately centrifuge sample at 1500 RCFs for 15 minutes, then pipette the supernatant plasma and transfer to a plastic transport tube.
4. Re-centrifuge plasma in the plastic transport tube at 1500 RCFs for 15 minutes to ensure that no red cells are present.
5. Pipette the top two-thirds of the supernatant plasma and transfer 2 mL plasma to another plastic transport tube.
Clearly label tube as citrate plasma.
6. Immediately freeze the plasma at -20 degrees C or lower for transport. Critical Frozen (CFZ).
7. When multiple tests are ordered, submit separate tube for this test.

Alternative Specimen

N/A