Benzodiazepines, Serum/Plasma

Test Code: TD_4299

CPT Code(s): 80346

$79

Test Details

Alternative Name(s)

Benzos, blood

Test Includes

Oxazepam
Temazepam
Nordiazepam
Lorazepam
Alprazolam
Alpha-Hydroxyalprazolam
Clonazepam
7-Aminoclonazepam
Midazolam
Diazepam
Alpha-Hydroxymidazolam
Chlordiazepoxide

Methodology

Liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Rejection Criteria

Samples collected in serum separator tube citrate (light blue top) whole blood or plasma avoid repeated freeze/thaw cycles hemolysis

Reference Range(s)

Negative

Specimen Requirements

Preferred Specimen

Sodium fluoride/potassium oxalate (gray top) tube

Minimum Volume

1 mL plasma or serum

Sample Stability

1 week room temperature
2 weeks refrigerated
3 years frozen

Transport Temperature

Refrigerated

Collection Instructions

2 mL sodium fluoride/potassium oxalate plasma.
Centrifuge sample and separate plasma from cells
immediately. Transfer plasma to a plastic transport tube.
Clearly label tube as sodium fluoride plasma.

Alternative Specimen

2 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tube as serum from a plain red top tube. Refrigerate. 2 mL EDTA (lavender top) or sodium heparin (green top) plasma. Centrifuge sample and separate plasma from cells immediately. Transfer plasma to a plastic transport tube. Clearly label tube as EDTA or heparin plasma. Refrigerate.