Indirect immunofluorescent assay (IFA) with human epithelial (hep-2) cell line substrate
Rejection Criteria
Hemolysis Lipemia
Reference Range(s)
Negative Will reflex to ANA titer and pattern if result is positive
Specimen Requirements
Preferred Specimen
Serum Separator Tube
Minimum Volume
1 mL serum
Sample Stability
1 day room temperature 1 week refrigerated
Transport Temperature
Refrigerated
Collection Instructions
Allow SST to clot in an upright position for
at least 30 minutes, then centrifuge sample within 2 hours
of collection.
Alternative Specimen
2 mL serum from a plain red top tube. Allow sample to
clot in an upright position for at least 60 minutes, then
centrifuge sample and transfer serum to a plastic transport
tube within 2 hours of collection. Clearly label tube as
serum from a plain red top tube.
