ANA Autoimmune Profile

Test Code: TD_4521

CPT Code(s): 86038 (If Positive Add 86039), 86235 X8, 86376, 86160 X2, 86431, 86225, 83516, 86200

$49

Test Details

Alternative Name(s)

N/A

Test Includes

Rheumatoid Factor, Quant 3505
Anti-Nuclear Antibodies 355000
Complement C3 3580
Complement C4 3585
DsDNA Antibody 4287
Thyroid Peroxidase AB 4513
Autoimmune Antibodies 452100
CCP IgG 5390

Methodology

Anti-nuclear antibodies:
indirect immunofluoresent assay (IFA) with human epithelial (hep-2) cell line substrate
Autoimmune antibodies, dsDNA antibody, ccp IgG:
multiplex flow immunoassay
Thyroid Peroxidase AB:
Roche Electrochemiluminescence Immunoassay (ECLIA)
complement c3, complement c4:
Roche COBAS immunoturbidimetric
Rheumatoid factor, quant:
Roche COBAS immunoturbidimetric
(WHO standard 64/2 traceable)

Rejection Criteria

Hemolysis
Avoid repeated freeze/thaw cycles

Reference Range(s)

By Report

Specimen Requirements

Preferred Specimen

Serum Separator Tube (SST)

Minimum Volume

4 mL serum

Sample Stability

Anti-nuclear antibodies, dsDNA antibody, ccp IgG:
1 day room temperature, 1 week refrigerated, 1 month frozen
Autoimmune antibodies:
1 week refrigerated, 6 months frozen
Thyroid Peroxidase AB:
8 days room temperature, 8 days refrigerated, 6 months frozen
Complement C3:
4 days room temperature, 8 days refrigerated, 8 days frozen
Complement C4:
2 days room temperature, 8 days refrigerated, 3 months frozen
Rheumatoid Factor, Quant:
1 day room temperature, 8 days refrigerated, 3 months frozen

Transport Temperature

Refrigerated

Collection Instructions

Collect 2 serum separator tubes. Allow tubes to clot in an upright position for at least 30 minutes, then centrifuge samples within 2 hours of collection. Transfer serum to a plastic transport tube. Refrigerate.
Note: Do not collect samples from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.

Alternative Specimen

Collect 2 plain red top tubes. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tubes as serum from a plain red top tube. Refrigerate.
Note: Do not collect samples from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.