Antinuclear Antibody with reflex Crithidia Luciliae
Test Includes
Anti-nuclear antibodies Autoimmune antibodies Thyroid peroxidase AB Complement C3 Complement C4 Rheumatoid factor, quant DNA ds Antibody Reflex Crithidia
Methodology
Anti-nuclear antibodies: Indirect Immunofluorescent Assay (IFA) with human epithelial (hep-2) cell line substrate autoimmune antibodies, CCP IgG DNA ds antibody: Multiplex Flow Immunoassay Thyroid peroxidase AB: Roche Electrochemiluminescence Immunoassay (ECLIA) Complement C3, Complement C4: Roche COBAS Immunoturbidimetric Rheumatoid factor, quant: Roche COBAS immunoturbidimetric (WHO standard 64/2 traceable)
Rejection Criteria
Moderate or greater hemolysis Avoid repeated freeze/thaw cycles
Reference Range(s)
By Report
Specimen Requirements
Preferred Specimen
Serum Seperator Tube
Minimum Volume
4 mL Serum
Sample Stability
Anti-nuclear antibodies, CCP IgG, DNA ds antibody: 1 day room temperature 1 week refrigerated 1 month frozen Autoimmune antibodies: 1 week refrigerated 6 months frozen Thyroid peroxidase AB: 8 days room temperature 8 days refrigerated 6 months frozen Complement C3: 4 days room temperature 8 days refrigerated 8 days frozen Complement C4: 2 days room temperature 8 days refrigerated 3 months frozen Rheumatoid factor, quant: 1 day room temperature 8 days refrigerated 3 months frozen
Transport Temperature
Refrigerated
Collection Instructions
Allow SST to clot in an upright position for at least 30 minutes, then centrifuge sample within 2 hours of collection. Refrigerate.
Alternative Specimen
Collect 2 plain red top tubes. Allow samples to clot in an upright position for at least 60 minutes, then centrifuge samples and transfer serum to 2 plastic transport tubes. Clearly label tubes as serum from a plain red top tube. Refrigerate. Note: Do not collect samples from patients receiving therapy with high biotin doses (>5 mg/day) until at least 8 hours following the last biotin administration.
