1. Collect baseline sample.
2 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample within 2 hours of collection. Clearly label tube as serum from a plain red top tube with time of collection and apply fluorescent timed collection label. Refrigerate.
2. Collect 2 mL serum from a plain red top tube for 1st, 2nd, 3rd, and 4th post specimens. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tube as serum from a plain red top tube with time of collection and apply fluorescent timed collection label. Refrigerate.
3. Submit baseline, 1st, 2nd, 3rd, and 4th specimens rubber banded together. Refrigerate.