ANA with Reflex Autoimmune AB Cascade

Test Code: TD_2677

CPT Code(s): 86038 If Level One Reflex Testing Performed, Add 86235 X3, 86255.
If Level 2 Reflex Testing Performed, Add 86235 X4.
If Level 3 Reflex Testing Performed, Add 83520 X2.

$84

Test Details

Alternative Name(s)

Antinuclear Antibody with Reflex Cascade

Test Includes

When ANA is Positive, will reflex to ANA Titer and Pattern, Chromatin IgG AB, dsDNA AB, RNP AB and sm AB as level one.
If all level one testing is Negative, Will reflex to Jo-1 AB, scl-70 AB, ssa/ssb AB as level two.
If all level one and level two testing is Negative, will reflex to Centromere B and Ribosomal P AB.
Additional charges will apply for all reflex testing performed.

Methodology

Indirect immunofluorescent assay (IFA) with human epithelial (hep-2) cell line substrate reflex antibody testing method is multiplex flow immunoassay.

Rejection Criteria

Hemolysis
Lipemia

Reference Range(s)

Negative

Specimen Requirements

Preferred Specimen

Serum Separator Tube

Minimum Volume

6 mL serum

Sample Stability

1 day room temperature
1 week refrigerated
1 month frozen

Transport Temperature

Refrigerated

Collection Instructions

Allow SST to clot in an upright position for
at least 30 minutes, then centrifuge sample within 2 hours
of collection

Alternative Specimen

6 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Clearly label tube as serum from a plain red top tube.