Cortisol, Free

Test Code: TD_5797
CPT Code(s): 82530

$225

Test Details

Alternative Name(s)

Cortisol, free, serum
Free Cortisol

Test Includes

Cortisol, Free

Methodology

Equilibrium dialysis/high performance liquid chromatography- tandem mass spectrometry (LC-MS/MS)

Rejection Criteria

Hemolysis
Lipemia
Icterus
Heparin (green top) plasma

Reference Range(s)

AM Sample: 0.21-1.04 UG/DL
PM Sample: 0.10-0.63 UG/DL

Specimen Requirements

Preferred Specimen

Serum separator tube

Minimum Volume

0.6 mL serum or plasma

Sample Stability

After separation from cells:
Unacceptable room temperature
1 week refrigerated
6 months frozen

Transport Temperature

Frozen

Collection Instructions

Allow SST to clot in an upright position for at least 30 minutes, then centrifuge sample within 2 hours of collection. Transfer serum to a plastic transport tube. Freeze.
Note: it is recommended to collect AM sample between 8-10 AM or PM sample between 4-6 PM. Clearly label tube with collection time.
When multiple tests are ordered, submit separate tube for this test.

Alternative Specimen

1 mL serum from a plain red top tube. Allow sample to clot in an upright position for at least 60 minutes, then centrifuge sample and transfer serum to a plastic transport tube within 2 hours of collection. Freeze.
Note: it is recommended to collect AM sample between 8-10 AM or PM sample between 4-6 PM. Clearly label tube with collection time. Clearly label tube as serum from a plain red top tube. When multiple tests are ordered, submit separate tube for this test.
1 mL EDTA (lavender top) plasma. Centrifuge sample and separate plasma from cells within 2 hours of collection. transfer plasma to a plastic transport tube. Freeze.
Note: it is recommended to collect AM sample between 8-10 AM or PM sample between 4-6 PM. Clearly label tube with collection time. Clearly label tube as serum from a plain red top tube.
When multiple tests are ordered, submit separate tube for this test.